LOCUS pCMV-Tol2 5693 bp DNA circular 27-OCT-2006 SOURCE ORGANISM COMMENT http://www.invitrogen.com/ COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|378918009| COMMENT VNTDBDATE|425386800| COMMENT LSOWNER| COMMENT VNTNAME|pCMV-Tol2| COMMENT VNTAUTHORNAME|Kirk Wangensteen| COMMENT VNTAUTHOREML|kirk@umn.edu| FEATURES Location/Qualifiers exon 11..1960 /vntifkey="61" /label=Tol2 rep_origin 2478..3358 /vntifkey="33" /label=ColE1 CDS complement(3419..4279) /vntifkey="4" /label=AP(R) /note="bla beta-lactamase, ampR ampicillin resistance" rep_origin 2661..2661 /vntifkey="33" /label=PMB1 /note="origin of replication" promoter 4876..5409 /vntifkey="29" /label=CMV\pro intron 5515..5674 /vntifkey="15" /label=splice\donor/acceptor polyA_signal 2010..2208 /vntifkey="25" /label=sv40p(A) primer_bind 4858..4873 /vntifkey="28" /label=M13\F primer_bind 2258..2274 /vntifkey="28" /label=M13\R BASE COUNT 1518 a 1366 c 1364 g 1445 t ORIGIN 1 ggccgccacc atggaggaag tatgtgattc atcagcagct gcgagcagca cagtccaaaa 61 tcagccacag gatcaagagc acccgtggcc gtatcttcgc gaattctttt ctttaagtgg 121 tgtaaataaa gattcattca agatgaaatg tgtcctctgt ctcccgctta ataaagaaat 181 atcggccttc aaaagttcgc catcaaacct aaggaagcat attgagagaa tgcacccaaa 241 ttacctcaaa aactactcta aattgacagc acagaagaga aagatcggga cctccaccca 301 tgcttccagc agtaagcaac tgaaagttga ctcagttttc ccagtcaaac atgtgtctcc 361 agtcactgtg aacaaagcta tattaaggta catcattcaa ggacttcatc ctttcagcac 421 tgttgatctg ccatcattta aagagctgat tagtacactg cagcctggca tttctgtcat 481 tacaaggcct actttacgct ccaagatagc tgaagctgct ctgatcatga aacagaaagt 541 gactgctgcc atgagtgaag ttgaatggat tgcaaccaca acggattgtt ggactgcacg 601 tagaaagtca ttcattggtg taactgctca ctggatcaac cctggaagtc ttgaaagaca 661 ttccgctgca cttgcctgca aaagattaat gggctctcat acttttgagg tactggccag 721 tgccatgaat gatatccact cagagtatga aatacgtgac aaggttgttt gcacaaccac 781 agacagtggt tccaacttta tgaaggcttt cagagttttt ggtgtggaaa acaatgatat 841 cgagactgag gcaagaaggt gtgaaagtga tgacactgat tctgaaggct gtggtgaggg 901 aagtgatggt gtggaattcc aagatgcctc acgagtcctg gaccaagacg atggcttcga 961 attccagcta ccaaaacatc aaaagtgtgc ctgtcactta cttaacctag tctcaagcgt 1021 tgatgcccaa aaagctctct caaatgaaca ctacaagaaa ctctacagat ctgtctttgg 1081 caaatgccaa gctttatgga ataaaagcag ccgatcggct ctagcagctg aagctgttga 1141 atcagaaagc cggcttcagc ttttaaggcc aaaccaaacg cggtggaatt caacttttat 1201 ggctgttgac agaattcttc aaatttgcaa agaagcagga gaaggcgcac ttcggaatat 1261 atgcacctct cttgaggttc caatgtttaa tccagcagaa atgctgttct tgacagagtg 1321 ggccaacaca atgcgtccag ttgcaaaagt actcgacatc ttgcaagcgg aaacgaatac 1381 acagctgggg tggctgctgc ctagtgtcca tcagttaagc ttgaaacttc agcgactcca 1441 ccattctctc aggtactgtg acccacttgt ggatgcccta caacaaggaa tccaaacacg 1501 attcaagcat atgtttgaag atcctgagat catagcagct gccatccttc tccctaaatt 1561 tcggacctct tggacaaatg atgaaaccat cataaaacga ggcatggact acatcagagt 1621 gcatctggag cctttggacc acaagaagga attggccaac agttcatctg atgatgaaga 1681 ttttttcgct tctttgaaac cgacaacaca tgaagccagc aaagagttgg atggatatct 1741 ggcctgtgtt tcagacacca gggagtctct gctcacgttt cctgctattt gcagcctctc 1801 tatcaagact aatacacctc ttcccgcatc ggctgcctgt gagaggcttt tcagcactgc 1861 aggattgctt ttcagcccca aaagagctag gcttgacact aacaattttg agaatcagct 1921 tctactgaag ttaaatctga ggttttacaa ctttgagtag actagtctga agggcgaatt 1981 ctgcagatat ccatcacact ggcggccgcg gggatccaga catgataaga tacattgatg 2041 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg 2101 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt 2161 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttcg gatcctctag 2221 agtcgacctg caggcatgca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa 2281 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 2341 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 2401 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 2461 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 2521 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 2581 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 2641 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 2701 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 2761 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 2821 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 2881 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 2941 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 3001 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 3061 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 3121 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 3181 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 3241 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 3301 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 3361 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 3421 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 3481 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 3541 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 3601 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 3661 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 3721 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 3781 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 3841 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 3901 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 3961 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 4021 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 4081 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 4141 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 4201 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 4261 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 4321 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 4381 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat 4441 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg 4501 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg 4561 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc 4621 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac 4681 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca 4741 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg 4801 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta 4861 aaacgacggc cagtgaattc gagcttgcat gcctgcaggt cgttacataa cttacggtaa 4921 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 4981 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 5041 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 5101 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 5161 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 5221 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 5281 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 5341 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 5401 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 5461 tccatagaag acaccgggac cgatccagcc tccggactct agaggatccg gtactcgagg 5521 aactgaaaaa ccagaaagtt aactggtaag tttagtcttt ttgtctttta tttcaggtcc 5581 cggatccggt ggtggtgcaa atcaaagaac tgctcctcag tggatgttgc ctttacttct 5641 aggcctgtac ggaagtgtta cttctgctct aaaagctgcg gaattgtacc cgc //