LOCUS pGEM\Tol2\PPTK_T 6285 bp DNA circular 9-FEB-2007 SOURCE ORGANISM COMMENT http://www.invitrogen.com/ COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|406309286| COMMENT VNTDBDATE|437653826| COMMENT LSOWNER| COMMENT VNTNAME|pGEM Tol2 PPTK_TS| COMMENT VNTAUTHORNAME|Karl J. Clark| COMMENT VNTOAUTHORNAME|Dan Carlson| COMMENT VNTOAUTHOREML|carl0246@umn.edu| COMMENT VNTOAUTHORAD1|1988 Fitch Ave| FEATURES Location/Qualifiers rep_origin complement(5650..6105) /vntifkey="33" /label=f1\Ori CDS 4607..5467 /vntifkey="4" /label=Amp primer_bind complement(244..268) /vntifkey="28" /label=Tol2-R3 misc_feature 3169..3360 /vntifkey="21" /label=Tol2\3' misc_feature complement(15..276) /vntifkey="21" /label=Tol2\5' CDS 779..2377 /vntifkey="4" /label=PuroDeltaTK primer complement(2624..2648) /vntifkey="27" /label=SENSE_PRM /note="Sense primer used for creating PCR PuroÆTK rc" promoter 356..693 /vntifkey="29" /label=MmPGK /note="Mus musculus PGK promoter (shortened 5' end- untested)." 5'UTR 688..772 /vntifkey="52" /label=PGK\5'\UTR polyA_signal 2404..2600 /vntifkey="25" /label=BGH(A) polyA_signal 2660..2864 /vntifkey="25" /label=SV40(A) polyA_signal 2865..3069 /vntifkey="25" /label=SV40(A) BASE COUNT 1465 a 1675 c 1641 g 1504 t ORIGIN 1 gggcgaattg ggcccagagg tgtaaagtac ttgagtaatt ttacttgatt actgtactta 61 agtattattt ttggggattt ttactttact tgagtacaat taaaaatcaa tacttttact 121 tttacttaat tacatttttt tagaaaaaaa agtacttttt actccttaca attttattta 181 cagtcaaaaa gtacttattt tttggagatc acttcattct attttccctt gctattacca 241 aaccaattga attgcgctga tgcccagttt aatttagctt ctgatagact aattgacatc 301 atttgagtca attggaggtg tacctgtgga tgtatttcaa gggatcctga gatctggcct 361 cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc 421 gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg 481 tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc 541 gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc 601 gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc 661 aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag 721 cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccat 781 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg 841 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg 901 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat 961 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggacag 1021 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc 1081 ccggctggcc gcgcagcaac agatggaagg cctcctgggc ggcgaccgac ccaaggagcc 1141 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag 1201 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga 1261 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga 1321 cgtcgagtgc ccgaaggacc gcgcgacctg gtgcatgacc cgcaaggccg gtgccggatc 1381 catgcccagc gtactgcggg tttatataga cggtcctcac gggatgggga aaaccaccac 1441 cacgcaactg ctggtggccc tgggttcgcg cgacgatatc gtctacgtac ccgagccgat 1501 gacttactgg caggtgctgg gggcttccga gacaatcgcg aacatctaca ccacacaaca 1561 ccgcctcgac cagggtgaga tatcggccgg ggacgcggcg gtggtaatga caagcgccca 1621 gataacaatg ggcatgcctt atgccgtgac cgacgccgtt ctggctcctc atatcggggg 1681 ggaggctggg agctcacatg ccccgccccc ggccctcacc ctcatcttcg accgccatcc 1741 catcgccgcc ctcctgtgct acccggccgc gcgatacctt atgggcagca tgacccccca 1801 ggccgtgctg gcgttcgtgg ccctcatccc gccgaccttg cccggcacaa acatcgtgtt 1861 gggggccctt ccggaggaga gacacatcga cgccctggcc aaacgccagc gccccggcga 1921 gcggcttgac ctggctatgc tggccgcgat tcgccgcgtt tacgggctgc ttgccaatac 1981 ggtgcggtat ctgcagggcg gcgggtcgtg gcgggaggat tggggacacg tttcggggac 2041 ggccgtgccg ccccagggtg ccgagcccca gagcaacgcg ggcccacgac cccatatcgg 2101 ggacacgtta tttaccctgt ttcgggcccc cgagttgctg gcccccaacg gcgacctgta 2161 taacgtgttt gcctgggcct tggacgtctt ggccaaacgc ctccgtccca tgcacgtctt 2221 tatcctggat tacgaccaat cgcccgccgg ctgccgggac gccctgctgc aacttacctc 2281 cgggatggtc cagacccacg tcaccacccc aggctccata ccgacgatct gcgacctggc 2341 gcgcacgttt gcccgggaga tgggggaggc taactgatct agagctcgct gatcagcctc 2401 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac 2461 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg 2521 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga 2581 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga 2641 aagaaccaaa gggcgaattc attgatgagt ttggacaaac cacaactaga atgcagtgaa 2701 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct 2761 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg 2821 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtcattga tgagtttgga 2881 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt 2941 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat 3001 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac 3061 aaatgtggtc attcagaatt cccatcacaa agctctgacc tcaatcctat agaaaggagg 3121 aatgagccaa aattcaccca acttattgtg ggaagctagc ttaaacaaga atctctagtt 3181 ttctttcttg cttttacttt tacttcctta atactcaagt acaattttaa tggagtactt 3241 ttttactttt actcaagtaa gattctagcc agatactttt acttttaatt gagtaaaatt 3301 ttccctaagt acttgtactt tcacttgagt aaaatttttg agtacttttt acacctctgc 3361 tcgaccatat gggagagctc ccaacgcgtt ggatgcatag cttgagtatt ctatagtgtc 3421 acctaaatag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc 3481 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat 3541 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc 3601 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg 3661 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 3721 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 3781 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 3841 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 3901 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 3961 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 4021 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 4081 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 4141 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 4201 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 4261 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc 4321 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 4381 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 4441 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 4501 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 4561 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 4621 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 4681 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 4741 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 4801 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 4861 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 4921 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 4981 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 5041 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 5101 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 5161 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 5221 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 5281 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 5341 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 5401 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 5461 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 5521 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 5581 cccgaaaagt gccacctgat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac 5641 cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa 5701 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat 5761 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg 5821 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac 5881 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta 5941 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag 6001 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg 6061 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtccatt cgccattcag 6121 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc 6181 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg 6241 acgttgtaaa acgacggcca gtgaattgta atacgactca ctata //