LOCUS pKC-PB 5303 bp DNA circular 10-AUG-2006 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|418380558| COMMENT VNTDBDATE|418380558| COMMENT LSOWNER| COMMENT VNTNAME|pKC-PB| COMMENT VNTAUTHORNAME|Karl J. Clark| FEATURES Location/Qualifiers CDS complement(4438..5247) /vntifkey="4" /label=Kan(R) rep_origin 3524..4405 /vntifkey="33" /label=ColE1\Ori polyA_signal 3107..3311 /vntifkey="25" /label=SV40(A) polyA_signal 3312..3516 /vntifkey="25" /label=SV40(A) enhancer 36..390 /vntifkey="9" /label=CMV-IE\enhancer promoter 391..672 /vntifkey="29" /label=Chicken\B-actin\promoter intron 766..920 /vntifkey="15" /label=mini-intron exon 673..765 /vntifkey="61" /label=non-coding\exon gene 999..3008 /vntifkey="60" /note="piggyBac ORF" CDS 1013..2794 /vntifkey="4" /label=piggyBac\Ts BASE COUNT 1496 a 1145 c 1209 g 1453 t ORIGIN 1 ctgagatctt aagggattac tagtttaaac ctagttatta atagtaatca attacggggt 61 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc 121 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag 181 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc 241 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg 301 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc 361 agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct 421 tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt attttttaat 481 tattttgtgc agcgatgggg gcgggggggg ggggggcgcg cgccaggcgg ggcggggcgg 541 ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg 601 ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc 661 gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc 721 tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac 781 ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg 841 tggctgcgtg aaagccttga ggggctccgg gagctagagc ctctgctaac catgttcatg 901 ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt 961 ttggcaaaga attttaagct agcgctaccg gactcagatc ctatataata aaatgggtag 1021 ttctttagac gatgagcata tcctctctgc tcttctgcaa agcgatgacg agcttgttgg 1081 tgaggattct gacagtgaaa tatcagatca cgtaagtgaa gatgacgtcc agagcgatac 1141 agaagaagcg tttatagatg aggtacatga agtgcagcca acgtcaagcg gtagtgaaat 1201 attagacgaa caaaatgtta ttgaacaacc aggttcttca ttggcttcta acagaatctt 1261 gaccttgcca cagaggacta ttagaggtaa gaataaacat tgttggtcaa cttcaaagtc 1321 cacgaggcgt agccgagtct ctgcactgaa cattgtcaga tctcaaagag gtccgacgcg 1381 tatgtgccgc aatatatatg acccactttt atgcttcaaa ctatttttta ctgatgagat 1441 aatttcggaa attgtaaaat ggacaaatgc tgagatatca ttgaaacgtc gggaatctat 1501 gacaggtgct acatttcgtg acacgaatga agatgaaatc tatgctttct ttggtattct 1561 ggtaatgaca gcagtgagaa aagataacca catgtccaca gatgacctct ttgatcgatc 1621 tttgtcaatg gtgtacgtct ctgtaatgag tcgtgatcgt tttgattttt tgatacgatg 1681 tcttagaatg gatgacaaaa gtatacggcc cacacttcga gaaaacgatg tatttactcc 1741 tgttagaaaa atatgggatc tctttatcca tcagtgcata caaaattaca ctccaggggc 1801 tcatttgacc atagatgaac agttacttgg ttttagagga cggtgtccgt ttaggatgta 1861 tatcccaaac aagccaagta agtatggaat aaaaatcctc atgatgtgtg acagtggtac 1921 gaagtatatg ataaatggaa tgccttattt gggaagagga acacagacca acggagtacc 1981 actcggtgaa tactacgtga aggagttatc aaagcctgtg cacggtagtt gtcgtaatat 2041 tacgtgtgac aattggttca cctcaatccc tttggcaaaa aacttactac aagaaccgta 2101 taagttaacc attgtgggaa ccgtgcgatc aaacaaacgc gagataccgg aagtactgaa 2161 aaacagtcgc tccaggccag tgggaacatc gatgttttgt tttgacggac cccttactct 2221 cgtctcatat aaaccgaagc cagctaagat ggtatactta ttatcatctt gtgatgagga 2281 tgcttctatc aacgaaagta ccggtaaacc gcaaatggtt atgtattata atcaaactaa 2341 aggcggagtg gacacgctag accaaatgtg ttctgtgatg acctgcagta ggaagacgaa 2401 taggtggcct atggcattat tgtacggaat gataaacatt gcctgcataa attcttttat 2461 tatatacagc cataatgtca gtagcaaggg agaaaaggtt caaagtcgca aaaaatttat 2521 gagaaacctt tacatgagcc tgacgtcatc gtttatgcgt aagcgtttag aagctcctac 2581 tttgaagaga tatttgcgcg ataatatctc taatattttg ccaaatgaag tgcctggtac 2641 atcagatgac agtactgaag agccagtaat gaaaaaacgt acttactgta cttactgccc 2701 ctctaaaata aggcgaaagg caaatgcatc gtgcaaaaaa tgcaaaaaag ttatttgtcg 2761 agagcataat attgatatgt gccaaagttg tttctgactg actaataagt ataatttgtt 2821 tctattatgt ataagttaag ctaattactt attttataat acaacatgac tgtttttaaa 2881 gtacaaaata agtttatttt tgtaaaagag agaatgttta aaagttttgt tactttagaa 2941 gaaattttga gtttttgttt ttttttaata aataaataaa cataaataaa ttgtttgttg 3001 aatttggatc tcgaggttcc cacaatggtt aattcgagct cgcccgggga tctaattcaa 3061 ttagagacta attcaattag agctaattca attaggatcc agaattcatt gatgagtttg 3121 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta 3181 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc 3241 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct 3301 acaaatgtgg tcattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc 3361 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa 3421 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag 3481 gttttttaaa gcaagtaaaa cctctacaaa tgtggtcatt cagctgttcc gcttcctcgc 3541 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 3601 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 3661 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 3721 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 3781 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 3841 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaaccgtg gcgctttctc 3901 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 3961 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 4021 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 4081 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 4141 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 4201 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 4261 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 4321 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 4381 aaaggatctt cacctagatc ctttttgcca gtgttacaac caattaacca attctgatta 4441 gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc 4501 atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag 4561 gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat 4621 taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga 4681 atccggtgag aatggcaaaa gtttatgcat ttctttccag acttgttcaa caggccagcc 4741 attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc 4801 ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg 4861 caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat caggatattc 4921 ttctaatacc tggaatgctg tttttccggg gatcgcagtg gtgagtaacc atgcatcatc 4981 aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag 5041 tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa 5101 ctctggcgca tcgggcttcc catacaagcg atagattgtc gcacctgatt gcccgacatt 5161 atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta atcgcggcct 5221 cgacgtttcc cgttgaatat ggctcataac accccttgta ttactgttta tgtaagcaga 5281 cagttttatt gttcatgatg cag //