LOCUS pKT2C-EGFP 4602 bp DNA circular 24-MAR-2005 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|369839169| COMMENT VNTDBDATE|369839169| COMMENT LSOWNER| COMMENT VNTNAME|pKT2C-EGFP| COMMENT VNTAUTHORNAME|Karl J. Clark| FEATURES Location/Qualifiers CDS complement(3738..4547) /vntifkey="4" /label=Kan(R) rep_origin 2824..3705 /vntifkey="33" /label=ColE1\Ori transposon 2571..2798 /vntifkey="44" /label=IR/DR(R) /note="IR/DR(R) has PCR induced mutations at bps 13 (T to A in the Ri), deleting bp 130 (ĘT), 143 (C to T) and 150 (C to A) [these bp designations correspond to the original defined IR/DR(R) from Ri to Ro, not the plasmid]" transposon complement(21..247) /vntifkey="44" /label=IR/DR(L) /note="IR/DR(L) appears to have a spontaneous mutation from A to T at bp 44 (happens to revert back to consensus) [the bp designation corresponds to the original defined IR/DR(L) from Lo to Li, not the plasmid]" enhancer 346..700 /vntifkey="9" /label=CMV-IE\enhancer promoter 701..982 /vntifkey="29" /label=Chicken\B-actin\promoter polyA_signal 2029..2473 /vntifkey="25" /label=Rabbit\B-globin\polyA intron 1076..1230 /vntifkey="15" /label=mini-intron exon 983..1075 /vntifkey="61" /label=non-coding\exon CDS 1304..2023 /vntifkey="4" /label=EGFP BASE COUNT 1172 a 1185 c 1078 g 1167 t ORIGIN 1 gctggatcca gatccctata cagttgaagt cggaagttta catacactta agttggagtc 61 attaaaactc gtttttcaac tactccacaa atttcttgtt aacaaacaat agttttggca 121 agtcagttag gacatctact ttgtgcatga cacaagtcat ttttccaaca attgtttaca 181 gacagattat ttcacttata attcactgta tcacaattcc agtgggtcag aagtttacat 241 acactaagtt gactgtgcct ttaaacagct tggaaaattc cagaaaatga tggtctccct 301 atagtgagtc gtattagatc ccgggattac tagtttaaac ctagttatta atagtaatca 361 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta 421 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat 481 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg 541 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac 601 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt 661 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc 721 acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt 781 attttttaat tattttgtgc agcgatgggg gcgggggggg ggggggcgcg cgccaggcgg 841 ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca 901 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa 961 aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc 1021 cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag 1081 cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt 1141 ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagctagagc ctctgctaac 1201 catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg 1261 tctcatcatt ttggcaaaga attcctcgag cctgaattct accatggtga gcaagggcga 1321 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca 1381 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa 1441 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac 1501 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa 1561 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa 1621 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct 1681 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta 1741 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt 1801 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa 1861 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc 1921 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac 1981 cgccgccggg atcactctcg gcatggacga gctgtacaag taaagatctt tttccctctg 2041 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga 2101 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat 2161 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat 2221 atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga 2281 aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt 2341 tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg 2401 ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc 2461 tcttatggag atccctcgac ctgcataccg gtcaagctag cgatatcaat taaccctcac 2521 taaagggaga ccaagttaaa caatttaaag gcaatgctac caaatactaa ttgagtgtat 2581 gtaaacttct gacccactgg gaatgtgatg aaagaaataa aagctgaaat gaatcattct 2641 ctctactatt attctgatat ttcacattct taaaataaag tggtgatcct aactgaccta 2701 agacagggaa tttttactag gattaaatgt caggaattgt gaaaaagtga gtttaaatgt 2761 atttggctaa ggtgtatgta aacttccgac ttcaactgta tagggatctg gtaccattta 2821 aatctgttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 2881 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 2941 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 3001 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 3061 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 3121 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 3181 gggaaccgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 3241 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 3301 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 3361 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 3421 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc 3481 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 3541 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 3601 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 3661 tttggtcatg agattatcaa aaaggatctt cacctagatc ctttttgcca gtgttacaac 3721 caattaacca attctgatta gaaaaactca tcgagcatca aatgaaactg caatttattc 3781 atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac 3841 tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt 3901 ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa 3961 tcaccatgag tgacgactga atccggtgag aatggcaaaa gtttatgcat ttctttccag 4021 acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg 4081 ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa 4141 ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt 4201 tcacctgaat caggatattc ttctaatacc tggaatgctg tttttccggg gatcgcagtg 4261 gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata 4321 aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct 4381 ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaagcg atagattgtc 4441 gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg 4501 ttggaattta atcgcggcct cgacgtttcc cgttgaatat ggctcataac accccttgta 4561 ttactgttta tgtaagcaga cagttttatt gttcatgatg ca //