LOCUS pKT2C-loxPTK-GFP 6995 bp DNA circular 29-APR-2006 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|386074141| COMMENT VNTDBDATE|408965854| COMMENT LSOWNER| COMMENT VNTNAME|pKT2C-loxPTK-GFP| COMMENT VNTAUTHORNAME|Karl J. Clark| FEATURES Location/Qualifiers CDS complement(6131..6940) /vntifkey="4" /label=Kan(R) rep_origin 5217..6098 /vntifkey="33" /label=ColE1\Ori transposon 4964..5191 /vntifkey="44" /label=IR/DR(R) /note="IR/DR(R) has PCR induced mutations at bps 13 (T to A in the Ri), deleting bp 130 (ĘT), 143 (C to T) and 150 (C to A) [these bp designations correspond to the original defined IR/DR(R) from Ri to Ro, not the plasmid]" transposon complement(21..247) /vntifkey="44" /label=IR/DR(L) /note="IR/DR(L) appears to have a spontaneous mutation from A to T at bp 44 (happens to revert back to consensus) [the bp designation corresponds to the original defined IR/DR(L) from Lo to Li, not the plasmid]" enhancer 346..700 /vntifkey="9" /label=CMV-IE\enhancer promoter 701..982 /vntifkey="29" /label=Chicken\B-actin\promoter polyA_signal 4422..4866 /vntifkey="25" /label=Rabbit\B-globin\polyA intron 1076..1230 /vntifkey="15" /label=mini-intron exon 983..1075 /vntifkey="61" /label=non-coding\exon CDS 3697..4416 /vntifkey="4" /label=EGFP misc_recomb 1291..1324 /vntifkey="86" /label=LoxP misc_recomb 3647..3680 /vntifkey="86" /label=LoxP polyA_signal 3221..3425 /vntifkey="25" /label=SV40(A) polyA_signal 3426..3630 /vntifkey="25" /label=SV40(A) CDS 1340..2938 /vntifkey="4" /label=PuroDeltaTK polyA_signal 2965..3161 /vntifkey="25" /label=BGH(A) BASE COUNT 1659 a 1907 c 1776 g 1653 t ORIGIN 1 gctggatcca gatccctata cagttgaagt cggaagttta catacactta agttggagtc 61 attaaaactc gtttttcaac tactccacaa atttcttgtt aacaaacaat agttttggca 121 agtcagttag gacatctact ttgtgcatga cacaagtcat ttttccaaca attgtttaca 181 gacagattat ttcacttata attcactgta tcacaattcc agtgggtcag aagtttacat 241 acactaagtt gactgtgcct ttaaacagct tggaaaattc cagaaaatga tggtctccct 301 atagtgagtc gtattagatc ccgggattac tagtttaaac ctagttatta atagtaatca 361 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta 421 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat 481 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg 541 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac 601 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt 661 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc 721 acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt 781 attttttaat tattttgtgc agcgatgggg gcgggggggg ggggggcgcg cgccaggcgg 841 ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca 901 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa 961 aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc 1021 cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag 1081 cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt 1141 ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagctagagc ctctgctaac 1201 catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg 1261 tctcatcatt ttggcaaaga attcgccctt ataacttcgt atagcataca ttatacgaag 1321 ttattggctg agatctacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga 1381 cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg 1441 ccacaccgtc gacccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct 1501 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc 1561 ggtctggacc acgccggaca gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg 1621 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggg 1681 cggcgaccga cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca 1741 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc 1801 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct 1861 cggcttcacc gtcaccgccg acgtcgagtg cccgaaggac cgcgcgacct ggtgcatgac 1921 ccgcaaggcc ggtgccggat ccatgcccag cgtactgcgg gtttatatag acggtcctca 1981 cgggatgggg aaaaccacca ccacgcaact gctggtggcc ctgggttcgc gcgacgatat 2041 cgtctacgta cccgagccga tgacttactg gcaggtgctg ggggcttccg agacaatcgc 2101 gaacatctac accacacaac accgcctcga ccagggtgag atatcggccg gggacgcggc 2161 ggtggtaatg acaagcgccc agataacaat gggcatgcct tatgccgtga ccgacgccgt 2221 tctggctcct catatcgggg gggaggctgg gagctcacat gccccgcccc cggccctcac 2281 cctcatcttc gaccgccatc ccatcgccgc cctcctgtgc tacccggccg cgcgatacct 2341 tatgggcagc atgacccccc aggccgtgct ggcgttcgtg gccctcatcc cgccgacctt 2401 gcccggcaca aacatcgtgt tgggggccct tccggaggag agacacatcg acgccctggc 2461 caaacgccag cgccccggcg agcggcttga cctggctatg ctggccgcga ttcgccgcgt 2521 ttacgggctg cttgccaata cggtgcggta tctgcagggc ggcgggtcgt ggcgggagga 2581 ttggggacac gtttcgggga cggccgtgcc gccccagggt gccgagcccc agagcaacgc 2641 gggcccacga ccccatatcg gggacacgtt atttaccctg tttcgggccc ccgagttgct 2701 ggcccccaac ggcgacctgt ataacgtgtt tgcctgggcc ttggacgtct tggccaaacg 2761 cctccgtccc atgcacgtct ttatcctgga ttacgaccaa tcgcccgccg gctgccggga 2821 cgccctgctg caacttacct ccgggatggt ccagacccac gtcaccaccc caggctccat 2881 accgacgatc tgcgacctgg cgcgcacgtt tgcccgggag atgggggagg ctaactgatc 2941 tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc 3001 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa 3061 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg 3121 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg 3181 ctctatggct tctgaggcgg aaagaaccaa agggcgaatt cattgatgag tttggacaaa 3241 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt 3301 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta 3361 tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat 3421 gtggtcattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt 3481 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt 3541 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt 3601 taaagcaagt aaaacctcta caaatgtggt cattcagcca ctcgagataa cttcgtatag 3661 catacattat acgaagttat aagggcgaat tctaccatgg tgagcaaggg cgaggagctg 3721 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc 3781 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc 3841 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc 3901 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc 3961 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag 4021 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc 4081 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc 4141 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc 4201 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc 4261 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg 4321 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc 4381 gggatcactc tcggcatgga cgagctgtac aagtaaagat ctttttccct ctgccaaaaa 4441 ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat 4501 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag 4561 ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca 4621 tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc 4681 ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat 4741 attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact 4801 agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg 4861 gagatccctc gacctgcata ccggtcaagc tagcgatatc aattaaccct cactaaaggg 4921 agaccaagtt aaacaattta aaggcaatgc taccaaatac taattgagtg tatgtaaact 4981 tctgacccac tgggaatgtg atgaaagaaa taaaagctga aatgaatcat tctctctact 5041 attattctga tatttcacat tcttaaaata aagtggtgat cctaactgac ctaagacagg 5101 gaatttttac taggattaaa tgtcaggaat tgtgaaaaag tgagtttaaa tgtatttggc 5161 taaggtgtat gtaaacttcc gacttcaact gtatagggat ctggtaccat ttaaatctgt 5221 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 5281 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 5341 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 5401 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 5461 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 5521 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaacc 5581 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 5641 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 5701 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 5761 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 5821 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc 5881 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 5941 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 6001 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 6061 atgagattat caaaaaggat cttcacctag atcctttttg ccagtgttac aaccaattaa 6121 ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag 6181 gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga 6241 ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat 6301 caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat 6361 gagtgacgac tgaatccggt gagaatggca aaagtttatg catttctttc cagacttgtt 6421 caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca 6481 ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct gttaaaagga caattacaaa 6541 caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg 6601 aatcaggata ttcttctaat acctggaatg ctgtttttcc ggggatcgca gtggtgagta 6661 accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg 6721 tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat 6781 gtttcagaaa caactctggc gcatcgggct tcccatacaa gcgatagatt gtcgcacctg 6841 attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat 6901 ttaatcgcgg cctcgacgtt tcccgttgaa tatggctcat aacacccctt gtattactgt 6961 ttatgtaagc agacagtttt attgttcatg atgca //