LOCUS pKT2P-NLS-Flp 4952 bp DNA circular 7-JUN-2007 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|367932115| COMMENT VNTDBDATE|448580030| COMMENT LSOWNER| COMMENT VNTNAME|pKT2P-NLS-Flp| COMMENT VNTAUTHORNAME|Dan Carlson| COMMENT VNTAUTHORTEL|612-624-3215| COMMENT VNTAUTHOREML|carll0246@umn.edu| FEATURES Location/Qualifiers CDS complement(4090..4899) /vntifkey="4" /label=Kan(R) rep_origin 3176..4057 /vntifkey="33" /label=ColE1\Ori transposon 2923..3150 /vntifkey="44" /label=IR/DR(R) /note="IR/DR(R) has PCR induced mutations at bps 13 (T to A in the Ri), deleting bp 130 (ĘT), 143 (C to T) and 150 (C to A) [these bp designations correspond to the original defined IR/DR(R) from Ri to Ro, not the plasmid]" transposon complement(23..249) /vntifkey="44" /label=IR/DR(L) /note="IR/DR(L) appears to have a spontaneous mutation from A to T at bp 44 (happens to revert back to consensus) [the bp designation corresponds to the original defined IR/DR(L) from Lo to Li, not the plasmid]" promoter 350..778 /vntifkey="29" /label=PGK\prom. intron 880..1034 /vntifkey="15" /label=mini-intron polyA_signal 2381..2825 /vntifkey="25" /label=Rabbit\B-globin\polyA CDS 1078..2373 /vntifkey="4" /label=Flp misc_signal 1084..1104 /vntifkey="87" /label=Large-T\NLS BASE COUNT 1385 a 1127 c 1110 g 1330 t ORIGIN 1 cagctggatc cagatcccta tacagttgaa gtcggaagtt tacatacact taagttggag 61 tcattaaaac tcgtttttca actactccac aaatttcttg ttaacaaaca atagttttgg 121 caagtcagtt aggacatcta ctttgtgcat gacacaagtc atttttccaa caattgttta 181 cagacagatt atttcactta taattcactg tatcacaatt ccagtgggtc agaagtttac 241 atacactaag ttgactgtgc ctttaaacag cttggaaaat tccagaaaat gatggtctcc 301 ctatagtgag tcgtattaga tcccgggatt actagtttaa acctagacac ggggttgggg 361 ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt 421 tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc 481 gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc 541 ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca 601 cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg 661 cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa 721 ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg 781 tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga 841 atcaccgacc tctctcgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct 901 tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt tctgtggctg 961 cgtgaaagcc ttgaggggct ccgggagcta gagcctctgc taaccatgtt catgccttct 1021 tctttttcct acagctcctg ggcaacgtgc tggttgttgt gctgtctcga ggccaccatg 1081 gctcccaaga agaagaggaa ggtgatgagt caatttgata tattatgtaa aacaccacct 1141 aaggtgcttg ttcgtcagtt tgtggaaagg tttgaaagac cttcaggtga gaaaatagca 1201 ttatgtgctg ctgaactaac ctatttatgt tggatgatta cacataacgg aacagcaatc 1261 aagagagcca cattcatgag ctataatact atcataagca attcgctgag tttggatatt 1321 gtcaacaagt cactgcagtt taaatacaag acgcaaaaag caacaattct ggaagcctca 1381 ttaaagaaat tgattcctgc ttgggaattt acaattattc cttactatgg acaaaaacat 1441 caatctgata tcactgatat tgtaagtagt ttgcaattac agttcgaatc atcggaagaa 1501 gcagataagg gaaatagcca cagtaaaaaa atgcttaaag cacttctaag tgagggtgaa 1561 agcatctggg agatcactga gaaaatacta aattcgtttg agtatacttc gagatttaca 1621 aaaacaaaaa ctttatacca attcctcttc ctagctactt tcatcaattg tggaagattc 1681 agcgatatta agaacgttga tccgaaatca tttaaattag tccaaaataa gtatctggga 1741 gtaataatcc agtgtttagt gacagagaca aagacaagcg ttagtaggca catatacttc 1801 tttagcgcaa ggggtaggat cgatccactt gtatatttgg atgaattttt gaggaattct 1861 gaaccagtcc taaaacgagt aaataggacc ggcaattctt caagcaacaa gcaggaatac 1921 caattattaa aagataactt agtcagatcg tacaacaaag ctttgaagaa aaatgcgcct 1981 tattcaatct ttgctataaa aaatggccca aaatctcaca ttggaagaca tttgatgacc 2041 tcatttcttt caatgaaggg cctaacggag ttgactaatg ttgtgggaaa ttggagcgat 2101 aagcgtgctt ctgccgtggc caggacaacg tatactcatc agataacagc aatacctgat 2161 cactacttcg cactagtttc tcggtactat gcatatgatc caatatcaaa ggaaatgata 2221 gcattgaagg atgagactaa tccaattgag gagtggcagc atatagaaca gctaaagggt 2281 agtgctgaag gaagcatacg ataccccgca tggaatggga taatatcaca ggaggtacta 2341 gactaccttt catcctacat aaatagacgc atataagatc tttttccctc tgccaaaaat 2401 tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt 2461 ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg 2521 gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat 2581 atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc 2641 cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata 2701 ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta 2761 gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg 2821 agatccctcg acctgcatac cggtcaagct agcgatatca attaaccctc actaaaggga 2881 gaccaagtta aacaatttaa aggcaatgct accaaatact aattgagtgt atgtaaactt 2941 ctgacccact gggaatgtga tgaaagaaat aaaagctgaa atgaatcatt ctctctacta 3001 ttattctgat atttcacatt cttaaaataa agtggtgatc ctaactgacc taagacaggg 3061 aatttttact aggattaaat gtcaggaatt gtgaaaaagt gagtttaaat gtatttggct 3121 aaggtgtatg taaacttccg acttcaactg tatagggatc tggtaccatt taaatctgtt 3181 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 3241 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 3301 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 3361 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 3421 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 3481 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaaccg 3541 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 3601 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 3661 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 3721 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 3781 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct 3841 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 3901 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 3961 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 4021 tgagattatc aaaaaggatc ttcacctaga tcctttttgc cagtgttaca accaattaac 4081 caattctgat tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg 4141 attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag 4201 gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc 4261 aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg 4321 agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc 4381 aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat 4441 tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac 4501 aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga 4561 atcaggatat tcttctaata cctggaatgc tgtttttccg gggatcgcag tggtgagtaa 4621 ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt 4681 cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg 4741 tttcagaaac aactctggcg catcgggctt cccatacaag cgatagattg tcgcacctga 4801 ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt 4861 taatcgcggc ctcgacgttt cccgttgaat atggctcata acaccccttg tattactgtt 4921 tatgtaagca gacagtttta ttgttcatga tg //