LOCUS pKT2P-PTK 5159 bp DNA circular 27-APR-2006 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|408794174| COMMENT VNTDBDATE|408794174| COMMENT LSOWNER| COMMENT VNTNAME|pKT2P-PTK| COMMENT VNTAUTHORNAME|Karl J. Clark| COMMENT VNTOAUTHORNAME|Fahrenkrug Lab| COMMENT VNTOAUTHORTEL|612-624-3215| COMMENT VNTOAUTHOREML|fahre001@umn.edu| COMMENT VNTOAUTHORAD1|Univeristy of Minnesota| COMMENT VNTOAUTHORAD2|495 AniSci/Vet Med| COMMENT VNTOAUTHORAD3|1988 Fitch Ave.| COMMENT VNTOAUTHORAD4|Saint Paul, MN 55303| FEATURES Location/Qualifiers CDS complement(4295..5104) /vntifkey="4" /label=Kan(R) rep_origin 3381..4262 /vntifkey="33" /label=ColE1\Ori transposon 3128..3355 /vntifkey="44" /label=IR/DR(R) /note="IR/DR(R) has PCR induced mutations at bps 13 (T to A in the Ri), deleting bp 130 (ÆT), 143 (C to T) and 150 (C to A) [these bp designations correspond to the original defined IR/DR(R) from Ri to Ro, not the plasmid]" transposon complement(21..247) /vntifkey="44" /label=IR/DR(L) /note="IR/DR(L) appears to have a spontaneous mutation from A to T at bp 44 (happens to revert back to consensus) [the bp designation corresponds to the original defined IR/DR(L) from Lo to Li, not the plasmid]" polyA_signal 2645..2849 /vntifkey="25" /label=SV40(A) polyA_signal 2850..3054 /vntifkey="25" /label=SV40(A) CDS 752..2362 /vntifkey="4" /label=PuroDeltaTK primer complement(2609..2633) /vntifkey="27" /label=SENSE_PRM /note="Sense primer used for creating PCR PuroÆTK rc" promoter 329..666 /vntifkey="29" /label=MmPGK /note="Mus musculus PGK promoter (shortened 5' end- untested)." 5'UTR 661..745 /vntifkey="52" /label=PGK\5'\UTR polyA_signal 2389..2585 /vntifkey="25" /label=BGH(A) BASE COUNT 1218 a 1416 c 1332 g 1193 t ORIGIN 1 gctggatcca gatccctata cagttgaagt cggaagttta catacactta agttggagtc 61 attaaaactc gtttttcaac tactccacaa atttcttgtt aacaaacaat agttttggca 121 agtcagttag gacatctact ttgtgcatga cacaagtcat ttttccaaca attgtttaca 181 gacagattat ttcacttata attcactgta tcacaattcc agtgggtcag aagtttacat 241 acactaagtt gactgtgcct ttaaacagct tggaaaattc cagaaaatga tggtctccct 301 atagtgagtc gtattagatc tgagatctgg cctcgcacac attccacatc caccggtagg 361 cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc 421 aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca 481 cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt 541 tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg 601 ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc 661 tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct 721 tcctcatctc cgggcctttc gacctgcagc caacgccacc atggggaccg agtacaagcc 781 cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc 841 gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt 901 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt 961 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gacagcgtcg aagcgggggc 1021 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca 1081 gcaacagatg gaaggcctcc tgggcggcga ccgacccaag gagcccgcgt ggttcctggc 1141 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc 1201 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg 1261 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg agtgcccgaa 1321 ggaccgcgcg acctggtgca tgacccgcaa ggccggtgcc ggatccatgc ccagcgtact 1381 gcgggtttat atagacggtc ctcacgggat ggggaaaacc accaccacgc aactgctggt 1441 ggccctgggt tcgcgcgacg atatcgtcta cgtacccgag ccgatgactt actggcaggt 1501 gctgggggct tccgagacaa tcgcgaacat ctacaccaca caacaccgcc tcgaccaggg 1561 tgagatatcg gccggggacg cggcggtggt aatgacaagc gcccagataa caatgggcat 1621 gccttatgcc gtgaccgacg ccgttctggc tcctcatatc gggggggagg ctgggagctc 1681 acatgccccg cccccggccc tcaccctcat cttcgaccgc catcccatcg ccgccctcct 1741 gtgctacccg gccgcgcgat accttatggg cagcatgacc ccccaggccg tgctggcgtt 1801 cgtggccctc atcccgccga ccttgcccgg cacaaacatc gtgttggggg cccttccgga 1861 ggagagacac atcgacgccc tggccaaacg ccagcgcccc ggcgagcggc ttgacctggc 1921 tatgctggcc gcgattcgcc gcgtttacgg gctgcttgcc aatacggtgc ggtatctgca 1981 gggcggcggg tcgtggcggg aggattgggg acacgtttcg gggacggccg tgccgcccca 2041 gggtgccgag ccccagagca acgcgggccc acgaccccat atcggggaca cgttatttac 2101 cctgtttcgg gcccccgagt tgctggcccc caacggcgac ctgtataacg tgtttgcctg 2161 ggccttggac gtcttggcca aacgcctccg tcccatgcac gtctttatcc tggattacga 2221 ccaatcgccc gccggctgcc gggacgccct gctgcaactt acctccggga tggtccagac 2281 ccacgtcacc accccaggct ccataccgac gatctgcgac ctggcgcgca cgtttgcccg 2341 ggagatgggg gaggctaact gatctagagc tcgctgatca gcctcgactg tgccttctag 2401 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac 2461 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca 2521 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag 2581 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccaaagggcg 2641 aattcattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt 2701 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta 2761 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt 2821 aaagcaagta aaacctctac aaatgtggtc attgatgagt ttggacaaac cacaactaga 2881 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc 2941 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt 3001 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtcattca 3061 gatcaattaa ccctcactaa agggagacca agttaaacaa tttaaaggca atgctaccaa 3121 atactaattg agtgtatgta aacttctgac ccactgggaa tgtgatgaaa gaaataaaag 3181 ctgaaatgaa tcattctctc tactattatt ctgatatttc acattcttaa aataaagtgg 3241 tgatcctaac tgacctaaga cagggaattt ttactaggat taaatgtcag gaattgtgaa 3301 aaagtgagtt taaatgtatt tggctaaggt gtatgtaaac ttccgacttc aactgtatag 3361 ggatctggta ccatttaaat ctgttccgct tcctcgctca ctgactcgct gcgctcggtc 3421 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 3481 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 3541 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 3601 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 3661 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 3721 tccgcctttc tcccttcggg aaccgtggcg ctttctcata gctcacgctg taggtatctc 3781 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 3841 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 3901 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 3961 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc 4021 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 4081 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 4141 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 4201 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 4261 tttgccagtg ttacaaccaa ttaaccaatt ctgattagaa aaactcatcg agcatcaaat 4321 gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa agccgtttct 4381 gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt 4441 ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa 4501 ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt 4561 tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac 4621 tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcgat 4681 cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca 4741 gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt 4801 ttccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga 4861 tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat 4921 cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat 4981 acaagcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat 5041 ataaatcagc atccatgttg gaatttaatc gcggcctcga cgtttcccgt tgaatatggc 5101 tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgatgca //