LOCUS pPBTP-PTK 5140 bp DNA circular 6-SEP-2006 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|420804408| COMMENT VNTDBDATE|420804408| COMMENT LSOWNER| COMMENT VNTNAME|pPBTP-PTK| COMMENT VNTAUTHORNAME|Karl J. Clark| FEATURES Location/Qualifiers CDS complement(4275..5084) /vntifkey="4" /label=Kan(R) rep_origin 3361..4242 /vntifkey="33" /label=ColE1\Ori LTR complement(19..256) /vntifkey="19" /label=LTR-1 /note="3' Terminal Repeat" LTR 3068..3338 /vntifkey="19" /label=LTR_2 /note="5' Terminal Repeat" polyA_signal 2614..2818 /vntifkey="25" /label=SV40(A) polyA_signal 2819..3023 /vntifkey="25" /label=SV40(A) CDS 721..2331 /vntifkey="4" /label=PuroDeltaTK primer complement(2578..2602) /vntifkey="27" /label=SENSE_PRM /note="Sense primer used for creating PCR PuroÆTK rc" promoter 298..635 /vntifkey="29" /label=MmPGK /note="Mus musculus PGK promoter (shortened 5' end- untested)." 5'UTR 630..714 /vntifkey="52" /label=PGK\5'\UTR polyA_signal 2358..2554 /vntifkey="25" /label=BGH(A) BASE COUNT 1222 a 1414 c 1319 g 1185 t ORIGIN 1 ctggatccca atccttaacc ctagaaagat aatcatattg tgacgtacgt taaagataat 61 catgcgtaaa attgacgcat gtgttttatc ggtctgtata tcgaggttta tttattaatt 121 tgaatagata ttaagtttta ttatatttac acttacatac taataataaa ttcaacaaac 181 aatttattta tgtttattta tttattaaaa aaaaacaaaa actcaaaatt tcttctataa 241 agtaacaaaa cttttatggt ctccctatag tgagtcgtat tagatcccct gagatctggc 301 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct 361 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt 421 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca 481 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct 541 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc 601 tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa 661 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc 721 aacgccacca tggggaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc 781 ccccgggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc 841 gtcgacccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc 901 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg 961 accacgccgg acagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc 1021 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct gggcggcgac 1081 cgacccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc 1141 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg 1201 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc 1261 accgtcaccg ccgacgtcga gtgcccgaag gaccgcgcga cctggtgcat gacccgcaag 1321 gccggtgccg gatccatgcc cagcgtactg cgggtttata tagacggtcc tcacgggatg 1381 gggaaaacca ccaccacgca actgctggtg gccctgggtt cgcgcgacga tatcgtctac 1441 gtacccgagc cgatgactta ctggcaggtg ctgggggctt ccgagacaat cgcgaacatc 1501 tacaccacac aacaccgcct cgaccagggt gagatatcgg ccggggacgc ggcggtggta 1561 atgacaagcg cccagataac aatgggcatg ccttatgccg tgaccgacgc cgttctggct 1621 cctcatatcg ggggggaggc tgggagctca catgccccgc ccccggccct caccctcatc 1681 ttcgaccgcc atcccatcgc cgccctcctg tgctacccgg ccgcgcgata ccttatgggc 1741 agcatgaccc cccaggccgt gctggcgttc gtggccctca tcccgccgac cttgcccggc 1801 acaaacatcg tgttgggggc ccttccggag gagagacaca tcgacgccct ggccaaacgc 1861 cagcgccccg gcgagcggct tgacctggct atgctggccg cgattcgccg cgtttacggg 1921 ctgcttgcca atacggtgcg gtatctgcag ggcggcgggt cgtggcggga ggattgggga 1981 cacgtttcgg ggacggccgt gccgccccag ggtgccgagc cccagagcaa cgcgggccca 2041 cgaccccata tcggggacac gttatttacc ctgtttcggg cccccgagtt gctggccccc 2101 aacggcgacc tgtataacgt gtttgcctgg gccttggacg tcttggccaa acgcctccgt 2161 cccatgcacg tctttatcct ggattacgac caatcgcccg ccggctgccg ggacgccctg 2221 ctgcaactta cctccgggat ggtccagacc cacgtcacca ccccaggctc cataccgacg 2281 atctgcgacc tggcgcgcac gtttgcccgg gagatggggg aggctaactg atctagagct 2341 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc 2401 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa 2461 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac 2521 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg 2581 gcttctgagg cggaaagaac caaagggcga attcattgat gagtttggac aaaccacaac 2641 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt 2701 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca 2761 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtca 2821 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa 2881 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca 2941 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca 3001 agtaaaacct ctacaaatgt ggtcattcag atcaattaac cctcactaaa gggagaccat 3061 aagttatcac gtaagtagaa catgaaataa caatataatt atcgtatgag ttaaatctta 3121 aaagtcacgt aaaagataat catgcgtcat tttgactcac gcggtcgtta tagttcaaaa 3181 tcagtgacac ttaccgcatt gacaagcacg cctcacggga gctccaagcg gcgactgaga 3241 tgtcctaaat gcacagcgac ggattcgcgc tatttagaaa gagagagcaa tatttcaaga 3301 atgcatgcgt caattttacg cagactatct ttctagggtt aatctaggta ccatttaaat 3361 ctgttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 3421 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 3481 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 3541 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 3601 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 3661 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 3721 aaccgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 3781 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 3841 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 3901 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 3961 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt 4021 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 4081 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 4141 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 4201 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt tttgccagtg ttacaaccaa 4261 ttaaccaatt ctgattagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata 4321 tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca 4381 ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca 4441 acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca 4501 ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc tttccagact 4561 tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta 4621 ttcattcgtg attgcgcctg agcgagacga aatacgcgat cgctgttaaa aggacaatta 4681 caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca 4741 cctgaatcag gatattcttc taatacctgg aatgctgttt ttccggggat cgcagtggtg 4801 agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat 4861 tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg 4921 ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaagcgata gattgtcgca 4981 cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg 5041 gaatttaatc gcggcctcga cgtttcccgt tgaatatggc tcataacacc ccttgtatta 5101 ctgtttatgt aagcagacag ttttattgtt catgatgcag //