LOCUS pPTn2P-PTK 5083 bp DNA circular 28-APR-2006 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|408927299| COMMENT VNTDBDATE|408927299| COMMENT LSOWNER| COMMENT VNTNAME|pPTn2P-PTK| COMMENT VNTAUTHORNAME|Karl J. Clark| FEATURES Location/Qualifiers CDS complement(4218..5027) /vntifkey="4" /label=Kan(R) rep_origin 3304..4185 /vntifkey="33" /label=ColE1\Ori misc_feature complement(12..227) /vntifkey="21" /label=IR/DR(L) misc_feature 3070..3286 /vntifkey="21" /label=IR/DR(R) polyA_signal 2604..2808 /vntifkey="25" /label=SV40(A) polyA_signal 2809..3013 /vntifkey="25" /label=SV40(A) CDS 711..2321 /vntifkey="4" /label=PuroDeltaTK primer complement(2568..2592) /vntifkey="27" /label=SENSE_PRM /note="Sense primer used for creating PCR PuroÆTK rc" promoter 288..625 /vntifkey="29" /label=MmPGK /note="Mus musculus PGK promoter (shortened 5' end- untested)." 5'UTR 620..704 /vntifkey="52" /label=PGK\5'\UTR polyA_signal 2348..2544 /vntifkey="25" /label=BGH(A) BASE COUNT 1181 a 1407 c 1325 g 1170 t ORIGIN 1 ctggatccga ttacagtgcc ttgcataagt attcaccccc tttggacttt tctacatttt 61 gtcatgctat aaccacagat taaaatttat ttcatcgtga gtttatgtaa tggaccaaca 121 caaaatagtg catcatttgg aagtgggggg aaatattaca tggatttcac aattatttac 181 aaataaaaat ctgaaaagtg ttgagtgcat atgtattcac cccctttact gtgaaacccc 241 taacaaagat ggtctcccta tagtgagtcg tattagatct gagatctggc ctcgcacaca 301 ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc 361 ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac 421 gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca 481 atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg 541 ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg 601 ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc 661 tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc aacgccacca 721 tggggaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg 781 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg 841 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg 901 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg 961 acagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg 1021 gttcccggct ggccgcgcag caacagatgg aaggcctcct gggcggcgac cgacccaagg 1081 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg 1141 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc 1201 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg 1261 ccgacgtcga gtgcccgaag gaccgcgcga cctggtgcat gacccgcaag gccggtgccg 1321 gatccatgcc cagcgtactg cgggtttata tagacggtcc tcacgggatg gggaaaacca 1381 ccaccacgca actgctggtg gccctgggtt cgcgcgacga tatcgtctac gtacccgagc 1441 cgatgactta ctggcaggtg ctgggggctt ccgagacaat cgcgaacatc tacaccacac 1501 aacaccgcct cgaccagggt gagatatcgg ccggggacgc ggcggtggta atgacaagcg 1561 cccagataac aatgggcatg ccttatgccg tgaccgacgc cgttctggct cctcatatcg 1621 ggggggaggc tgggagctca catgccccgc ccccggccct caccctcatc ttcgaccgcc 1681 atcccatcgc cgccctcctg tgctacccgg ccgcgcgata ccttatgggc agcatgaccc 1741 cccaggccgt gctggcgttc gtggccctca tcccgccgac cttgcccggc acaaacatcg 1801 tgttgggggc ccttccggag gagagacaca tcgacgccct ggccaaacgc cagcgccccg 1861 gcgagcggct tgacctggct atgctggccg cgattcgccg cgtttacggg ctgcttgcca 1921 atacggtgcg gtatctgcag ggcggcgggt cgtggcggga ggattgggga cacgtttcgg 1981 ggacggccgt gccgccccag ggtgccgagc cccagagcaa cgcgggccca cgaccccata 2041 tcggggacac gttatttacc ctgtttcggg cccccgagtt gctggccccc aacggcgacc 2101 tgtataacgt gtttgcctgg gccttggacg tcttggccaa acgcctccgt cccatgcacg 2161 tctttatcct ggattacgac caatcgcccg ccggctgccg ggacgccctg ctgcaactta 2221 cctccgggat ggtccagacc cacgtcacca ccccaggctc cataccgacg atctgcgacc 2281 tggcgcgcac gtttgcccgg gagatggggg aggctaactg atctagagct cgctgatcag 2341 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct 2401 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc 2461 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg 2521 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg 2581 cggaaagaac caaagggcga attcattgat gagtttggac aaaccacaac tagaatgcag 2641 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata 2701 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg 2761 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtca ttgatgagtt 2821 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc 2881 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat 2941 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct 3001 ctacaaatgt ggtcattcag atcaattaac cctcactaaa gggagaccag gttctaccaa 3061 gtattgacac aggggggtga atacttatgc acccaacaga tgtcaacttt tttgttctca 3121 ttattgtttg tgtcacaata aaatttattt tgcacctcca aagtactatg catgttttgt 3181 tgatcaaacg ggaaaaagtt tatttaagtc tatttgaatt ccagttagta acagtacata 3241 atgggaaaaa gtccaagggg ggtgaatact tatgcaaggc actgtaatcg gtaccattta 3301 aatctgttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 3361 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 3421 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 3481 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 3541 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 3601 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 3661 gggaaccgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 3721 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 3781 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 3841 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 3901 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc 3961 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 4021 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 4081 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 4141 tttggtcatg agattatcaa aaaggatctt cacctagatc ctttttgcca gtgttacaac 4201 caattaacca attctgatta gaaaaactca tcgagcatca aatgaaactg caatttattc 4261 atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac 4321 tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt 4381 ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa 4441 tcaccatgag tgacgactga atccggtgag aatggcaaaa gtttatgcat ttctttccag 4501 acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg 4561 ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa 4621 ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt 4681 tcacctgaat caggatattc ttctaatacc tggaatgctg tttttccggg gatcgcagtg 4741 gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata 4801 aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct 4861 ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaagcg atagattgtc 4921 gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg 4981 ttggaattta atcgcggcct cgacgtttcc cgttgaatat ggctcataac accccttgta 5041 ttactgttta tgtaagcaga cagttttatt gttcatgatg cag //