LOCUS pT2-FLoxP-PTK 6186 bp DNA circular 1-JUN-2007 DEFINITION Construction option #4 of pT2-FRTLoxP-PurodeltaTK-FRTLoxP. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|353501349| COMMENT VNTDBDATE|448051746| COMMENT LSOWNER| COMMENT VNTNAME|pT2-FLoxP-PTK| COMMENT VNTAUTHORNAME|Karl J. Clark| COMMENT VNTOAUTHORNAME|Demo User| COMMENT VNTREPLTYPE|Plasmid COMMENT VNTEXTCHREPL|Bacteria FEATURES Location/Qualifiers CDS 3158..3385 /vntifkey="4" /label=IR/DR(R) /note="IR/DR(R) T2 version has PCR induced mutations at bps 13 (T to A in the Ri), deleting bp 130 (ĘT), 143 (C to T) and 150 (C to A) [these bp designations correspond to the original defined IR/DR(R) from Ri to Ro, not the plasmid]" CDS complement(118..344) /vntifkey="4" /label=IR/DR(L) /note="IR/DR(L) T2 version" promoter complement(3489..3611) /vntifkey="30" /label=LacZ\promoter rep_origin 3705..4585 /vntifkey="33" /label=ColE\Ori CDS complement(4649..5506) /vntifkey="4" /label=Amp rep_origin 5637..6093 /vntifkey="33" /label=F1\Ori promoter complement(2511..2939) /vntifkey="29" /label=MmPGK /note="Mus musculus PGK promoter" polyA_signal complement(529..806) /vntifkey="25" /label=BPA CDS complement(809..2419) /vntifkey="4" /label=PuroDeltaTK misc_feature 431..478 /vntifkey="21" /label=FRT misc_feature 3027..3074 /vntifkey="21" /label=FRT misc_feature 479..512 /vntifkey="21" /label=LoxP misc_feature 2993..3026 /vntifkey="21" /label=LoxP 5'UTR complement(2426..2510) /vntifkey="52" /label=PGK\5'UTR BASE COUNT 1399 a 1661 c 1705 g 1421 t ORIGIN 1 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc 61 cagtgagcgc gcgtaatacg actcactata gggcgaattg gagctcggat ccctatacag 121 ttgaagtcgg aagtttacat acacttaagt tggagtcatt aaaactcgtt tttcaactac 181 tccacaaatt tcttgttaac aaacaatagt tttggcaagt cagttaggac atctactttg 241 tgcatgacac aagtcatttt tccaacaatt gtttacagac agattatttc acttataatt 301 cactgtatca caattccagt gggtcagaag tttacataca ctaagttgac tgtgccttta 361 aacagcttgg aaaattccag aaaatgatgt catggcttta gaagctagat ctggccatct 421 agagcggccg gaagttccta ttccgaagtt cctattctct agaaagtata ggaacttcat 481 aacttcgtat aatgtatgct atacgaagtt atctcgagaa ttccctcgag ccccagctgg 541 ttctttccgc ctcagaagcc atagagccca ccgcatcccc agcatgcctg ctattgtctt 601 cccaatcctc ccccttgctg tcctgcccca ccccaccccc cagaatagaa tgacacctac 661 tcagacaatg cgatgcaatt tcctcatttt attaggaaag gacagtggga gtggcacctt 721 ccagggtcaa ggaaggcacg ggggaggggc aaacaacaga tggctggcaa ctagaaggca 781 cagtcgaggc tgatcagcga gctctagatc agttagcctc ccccatctcc cgggcaaacg 841 tgcgcgccag gtcgcagatc gtcggtatgg agcctggggt ggtgacgtgg gtctggacca 901 tcccggaggt aagttgcagc agggcgtccc ggcagccggc gggcgattgg tcgtaatcca 961 ggataaagac gtgcatggga cggaggcgtt tggccaagac gtccaaggcc caggcaaaca 1021 cgttatacag gtcgccgttg ggggccagca actcgggggc ccgaaacagg gtaaataacg 1081 tgtccccgat atggggtcgt gggcccgcgt tgctctgggg ctcggcaccc tggggcggca 1141 cggccgtccc cgaaacgtgt ccccaatcct cccgccacga cccgccgccc tgcagatacc 1201 gcaccgtatt ggcaagcagc ccgtaaacgc ggcgaatcgc ggccagcata gccaggtcaa 1261 gccgctcgcc ggggcgctgg cgtttggcca gggcgtcgat gtgtctctcc tccggaaggg 1321 cccccaacac gatgtttgtg ccgggcaagg tcggcgggat gagggccacg aacgccagca 1381 cggcctgggg ggtcatgctg cccataaggt atcgcgcggc cgggtagcac aggagggcgg 1441 cgatgggatg gcggtcgaag atgagggtga gggccggggg cggggcatgt gagctcccag 1501 cctccccccc gatatgagga gccagaacgg cgtcggtcac ggcataaggc atgcccattg 1561 ttatctgggc gcttgtcatt accaccgccg cgtccccggc cgatatctca ccctggtcga 1621 ggcggtgttg tgtggtgtag atgttcgcga ttgtctcgga agcccccagc acctgccagt 1681 aagtcatcgg ctcgggtacg tagacgatat cgtcgcgcga acccagggcc accagcagtt 1741 gcgtggtggt ggttttcccc atcccgtgag gaccgtctat ataaacccgc agtacgctgg 1801 gcatggatcc ggcaccggcc ttgcgggtca tgcaccaggt cgcgcggtcc ttcgggcact 1861 cgacgtcggc ggtgacggtg aagccgagcc gctcgtagaa ggggaggttg cggggcgcgg 1921 aggtctccag gaaggcgggc accccggcgc gctcggccgc ctccactccg gggagcacga 1981 cggcgctgcc cagacccttg ccctggtggt cgggcgagac gccgacggtg gccaggaacc 2041 acgcgggctc cttgggtcgg tcgccgccca ggaggccttc catctgttgc tgcgcggcca 2101 gccgggaacc gctcaactcg gccatgcgcg ggccgatctc ggcgaacacc gcccccgctt 2161 cgacgctgtc cggcgtggtc cagaccgcca ccgcggcgcc gtcgtccgcg acccacacct 2221 tgccgatgtc gagcccgacg cgcgtgagga agagttcttg cagctcggtg acccgctcga 2281 tgtggcggtc cgggtcgacg gtgtggcgcg tggcggggta gtcggcgaac gcggcggcga 2341 gggtgcgtac ggcccggggg acgtcgtcgc gggtggcgag gcgcaccgtg ggcttgtact 2401 cggtccccat ggtggcgttg gctgcaggtc gaaaggcccg gagatgagga agaggagaac 2461 agcgcggcag acgtgcgctt ttgaagcgtg cagaatgccg ggcctccgga ggaccttcgg 2521 gcgcccgccc cgcccctgag cccgcccctg agcccgcccc cggacccacc ccttcccagc 2581 ctctgagccc agaaagcgaa ggagcaaagc tgctattggc cgctgcccca aaggcctacc 2641 cgcttccatt gctcagcggt gctgtccatc tgcacgagac tagtgagacg tgctacttcc 2701 atttgtcacg tcctgcacga cgcgagctgc ggggcggggg ggaacttcct gactagggga 2761 ggagtagaag gtggcgcgaa ggggccacca aagaacggag ccggttggcg cctaccggtg 2821 gatgtggaat gtgtgcgagg ccagaggcca cttgtgtagc gccaagtgcc cagcggggct 2881 gctaaagcgc atgctccaga ctgccttggg aaaagcgcct cccctacccg gtagaattcg 2941 atatcaagct tatcgatacc ggcgacctcg agggaggcct actagtccat ggataacttc 3001 gtataatgta tgctatacga agttatgaag ttcctattcc gaagttccta ttctctagaa 3061 agtataggaa cttctcatgg atatcaagct tgtggaaggc tactcgaaat gtttgaccca 3121 agttaaacaa tttaaaggca atgctaccaa atactaattg agtgtatgta aacttctgac 3181 ccactgggaa tgtgatgaaa gaaataaaag ctgaaatgaa tcattctctc tactattatt 3241 ctgatatttc acattcttaa aataaagtgg tgatcctaac tgacctaaga cagggaattt 3301 ttactaggat taaatgtcag gaattgtgaa aaagtgagtt taaatgtatt tggctaaggt 3361 gtatgtaaac ttccgacttc aactgtatag ggatcctcta gctagagtcg acctcgaggg 3421 ggggcccggt acccagcttt tgttcccttt agtgagggtt aatttcgagc ttggcgtaat 3481 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 3541 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa 3601 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat 3661 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 3721 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 3781 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 3841 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 3901 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 3961 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 4021 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 4081 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 4141 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 4201 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 4261 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 4321 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 4381 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 4441 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 4501 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 4561 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 4621 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 4681 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 4741 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 4801 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 4861 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 4921 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 4981 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 5041 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 5101 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 5161 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 5221 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 5281 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 5341 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 5401 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 5461 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 5521 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 5581 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 5641 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta 5701 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt 5761 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg 5821 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat 5881 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac 5941 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag 6001 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg 6061 cgaattttaa caaaatatta acgcttacaa tttccattcg ccattcaggc tgcgcaactg 6121 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg 6181 tgctgc //