(1) Screening the INRA Porcine Somatic Cell Panel. We suggest that investigators first demonstrate the utility of their individual markers to be mapped by genotyping DNA from the INRA porcine somatic cell panel and control rodent and porcine DNA. This step will assist investigators in improving the conditions for genotyping and also provide a provisional chromosomal assignment.
(2) Distribution of the IMpRH will be in aliquots of 1.5 micro grams of DNA from each of the 118 hybrids, which will permit 30 duplicate genotypes (25 ng/reaction). This panel must be used in full. Radiation hybrid genotypes and primer sequences must be submitted to the database within 1 calendar year and prior to receiving additional DNA. In all cases, genotypes must be submitted at the time of publication. Please note the University of Minnesota and INRA will provide genotyping if ten or less markers are to be used.
(3) Submission of genotypes must be made electronically directly into the INRA database. Current map assignments and information regarding markers are provided at the University of Minnesota website. Instructions for submission can be obtained from either website, which are linked. To improve characterization of the IMpRH, data will be made available publicly at the time of publication or within one year or after submission to the database. During this period, data confidentiality will be maintained.
(4) A Material Transfer Agreement must be completed prior to the shipment of IMpRH Panel DNA. Contact information is provided below. The MTA can be downloaded from this website. A hardcopy with institutional signatures is required prior to the distribution of DNA.